4CCG
Structure of an E2-E3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 109.224, 109.224, 117.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.817 - 2.400 |
| R-factor | 0.2141 |
| Rwork | 0.212 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1YH2 3k1l |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.670 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.820 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.750 |
| Number of reflections | 28506 | |
| <I/σ(I)> | 5.7 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.6M AMMOMIUM SULPHATE, 0.1M HEPES PH7.5, 0.2M NACL |
