4CBM
Pestivirus NS3 helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-14 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.100, 159.585, 99.227 |
Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.270 |
R-factor | 0.2355 |
Rwork | 0.233 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cbg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.980 | 3.450 |
High resolution limit [Å] | 3.270 | 3.270 |
Rmerge | 0.130 | 0.550 |
Number of reflections | 31533 | |
<I/σ(I)> | 7 | 1.9 |
Completeness [%] | 94.4 | 91.7 |
Redundancy | 2.4 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 0.1 M NAAC, 0.025 M CALCIUM ACETATE, 9 % PEG 4000, pH 4.6 |