4CB6
Structure of Influenza A H5N1 PB2 cap-binding domain with bound cap analogue (compound 11)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.170, 31.130, 98.790 |
| Unit cell angles | 90.00, 118.27, 90.00 |
Refinement procedure
| Resolution | 45.410 - 1.900 |
| R-factor | 0.21466 |
| Rwork | 0.211 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.615 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 24866 | |
| <I/σ(I)> | 8.6 | 2.6 |
| Completeness [%] | 98.8 | 98.5 |
| Redundancy | 2.95 | 2.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 0.2 M AMMONIUM IODIDE, PH 6.3, 20% PEG 3350 |
