4CA1
Crystal structure of Siah1 at 1.58 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 287 |
| Detector technology | PIXEL |
| Collection date | 2012-12-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 76.060, 104.120, 133.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.110 - 1.580 |
| R-factor | 0.14315 |
| Rwork | 0.141 |
| R-free | 0.18907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c9z |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.335 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.060 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 72738 | |
| <I/σ(I)> | 16.1 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | CRYSTALS GREW FROM EQUAL VOLUMES OF PROTEIN SOLUTION (15 MG ML-1 IN 15 MM TRIS-HCL PH7.5, 30 MM NACL AND 10 MM DTT) AND RESERVOIR SOLUTION (100 MM HEPES PH7.0, 1.45 LI2SO4). |
