4C9Q
Structure of yeast mitochondrial ADP/ATP carrier isoform 3 inhibited by carboxyatractyloside (P21 crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.695, 107.876, 57.002 |
| Unit cell angles | 90.00, 93.62, 90.00 |
Refinement procedure
| Resolution | 41.449 - 3.200 |
| R-factor | 0.2596 |
| Rwork | 0.258 |
| R-free | 0.29480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c9j |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.768 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.450 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.130 | 0.410 |
| Number of reflections | 11131 | |
| <I/σ(I)> | 5.7 | 2.5 |
| Completeness [%] | 98.0 | 98.3 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH 8.5, 5MM SODIUM CITRATE, 11% T-BUTANOL, 26% PEG400 |
