4C7O
The structural basis of FtsY recruitment and GTPase activation by SRP RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2011-11-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.720, 166.330, 154.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.222 - 2.600 |
| R-factor | 0.1657 |
| Rwork | 0.163 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cnw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.220 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.770 |
| Number of reflections | 45637 | |
| <I/σ(I)> | 13.38 | 2.77 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 4.28 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
