4C7G
Structure and activity of the GH20 beta-N-acetylhexosaminidase from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Collection date | 2012-05-19 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.170, 64.450, 143.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.830 - 1.800 |
| R-factor | 0.16926 |
| Rwork | 0.167 |
| R-free | 0.21006 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c7d |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.814 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.350 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.510 |
| Number of reflections | 50954 | |
| <I/σ(I)> | 12.2 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.0, 4 % PEG 6000 |
