4C7D
Structure and activity of the GH20 beta-N-acetylhexosaminidase from Streptomyces coelicolor A3(2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2011-07-11 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.660, 128.900, 150.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 97.850 - 1.850 |
R-factor | 0.16465 |
Rwork | 0.163 |
R-free | 0.20231 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hp4 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.888 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.240 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.060 | 0.560 |
Number of reflections | 120786 | |
<I/σ(I)> | 16.8 | 3.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M HEPES PH 7.3, 8 % (W/V) PEG 6000 |