4C53
Crystal Structure of Guanarito virus GP2 in the post-fusion conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 |
| Unit cell lengths | 98.500, 98.500, 78.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.440 - 4.140 |
| R-factor | 0.256 |
| Rwork | 0.255 |
| R-free | 0.27580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mko |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.140 |
| High resolution limit [Å] | 4.140 | 4.140 |
| Rmerge | 0.090 | 1.000 |
| Number of reflections | 5894 | |
| <I/σ(I)> | 26.6 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5 | 0.15 M LI2SO4, 0.1 M CITRIC ACID PH 3.5, 18% W/V PEG 6000 |
