4C2Z
Human N-myristoyltransferase (NMT1) with Myristoyl-CoA and inhibitor bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.630, 179.120, 58.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.560 - 2.080 |
| R-factor | 0.17273 |
| Rwork | 0.169 |
| R-free | 0.23814 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.958 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.000 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.150 | 0.690 |
| Number of reflections | 50468 | |
| <I/σ(I)> | 12.9 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 25% PEG 2000 MME, 0.2 M KBR, 100 MM NA CITRATE PH 4.5, 5% GLYCEROL |
