4C2M

Structure of RNA polymerase I at 2.8 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1
Unit cell lengths	122.740, 139.020, 209.550
Unit cell angles	108.06, 95.40, 93.85

Refinement procedure

Resolution	39.292 - 2.800
R-factor	0.1697
Rwork	0.169
R-free	0.20990
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.241
Data reduction software	XDS
Data scaling software	XDS
Phasing software	autoSHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.240
Number of reflections	320814
<I/σ(I)>	9.61	1.52
Completeness [%]	99.9	99.8
Redundancy	13.1	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		10% PEG4000, 300MM AMMONIUM ACETATE, 50MM HEPES PH 7.5, 5MM TCEP
1		7		10% PEG4000, 300MM AMMONIUM ACETATE, 50MM HEPES PH 7.5, 5MM TCEP