4C20
L-Fucose Isomerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-13 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 151.931, 151.931, 408.853 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.070 - 2.410 |
R-factor | 0.15596 |
Rwork | 0.154 |
R-free | 0.19045 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fui |
RMSD bond length | 0.010 |
RMSD bond angle | 1.397 |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.540 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.080 | 0.380 |
Number of reflections | 70320 | |
<I/σ(I)> | 24.2 | 6.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.7 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | SPFCSI AT A CONCENTRATION OF 12 MG ML-1, BUFFERED IN 20 MM TRIS-HCL (PH 7.0), 150 MM NACL, AND 2 MM DTT WAS CRYSTALLIZED BY MIXING WITH EQUAL VOLUMES OF 100 MM TRIS-HCL (PH 8.0), 27% (W PER V) POLYETHYLENE GLYCOL 4,000, 50 MM NACL, AND 200 MM MAGNESIUM SULPHATE |