4C11
Dengue virus RNA dependent RNA polymerase with residues from the NS5 linker region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 122.990, 136.050, 103.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.600 |
| R-factor | 0.1947 |
| Rwork | 0.192 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.120 |
| Number of reflections | 53052 |
| <I/σ(I)> | 11.4 |
| Completeness [%] | 98.5 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
