4C0A
Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-10 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 90.980, 65.780, 196.860 |
Unit cell angles | 90.00, 96.13, 90.00 |
Refinement procedure
Resolution | 42.840 - 3.300 |
R-factor | 0.2013 |
Rwork | 0.199 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1R8S AND 3QWM |
RMSD bond length | 0.010 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.030 | 0.090 |
Number of reflections | 67437 | |
<I/σ(I)> | 6.8 | 1.94 |
Completeness [%] | 99.0 | 95.6 |
Redundancy | 3.24 | 3.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.15 M AMMONIUM SULFATE, 0.1 M MES PH 6 AND 16% PEG 4000 |