4C02
Crystal structure of human ACVR1 (ALK2) in complex with FKBP12.6 and dorsomorphin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 182.330, 182.330, 182.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.820 - 2.170 |
| R-factor | 0.17833 |
| Rwork | 0.177 |
| R-free | 0.19754 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1C9H AND 3H9R |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.767 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.230 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.170 | 1.070 |
| Number of reflections | 169113 | |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 1.8M AMMONIUM CITRATE, pH 7.2 |
