4BY6
Yeast Not1-Not2-Not5 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-16 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.452, 109.174, 133.622 |
| Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
| Resolution | 48.904 - 2.797 |
| R-factor | 0.1834 |
| Rwork | 0.181 |
| R-free | 0.22580 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.117 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.150 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 77882 | |
| <I/σ(I)> | 17.3 | 2.9 |
| Completeness [%] | 97.0 | 97 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 6.5% (W/V) PEG 8000, 100 MM MES 6.5, 200 MM CALCIUM ACETATE | ||
| 1 | 6.5 | 6.5% (W/V) PEG 8000, 100 MM MES 6.5, 200 MM CALCIUM ACETATE |
