4BX7

trans-divalent streptavidin bound to biotin-4-fluorescein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-07
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	64.410, 64.410, 103.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.064 - 2.260
R-factor	0.1863
Rwork	0.184
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ry1
RMSD bond length	0.006
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.060	2.340
High resolution limit [Å]	2.260	2.260
Rmerge	0.080	0.750
Number of reflections	12059
<I/σ(I)>	18.95	3.26
Completeness [%]	99.8	99.4
Redundancy	9.6	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	65% V/V MPD, 0.1 M MES PH 4.0. CRYSTALS WERE OBTAINED BY THE SITTING-DROP VAPOR-DIFFUSION METHOD AT 277 K, REACHED A MAXIMUM SIZE AFTER 14 DAYS AND WERE HARVESTED SOON AFTER