4BWS

Crystal structure of the heterotrimer of PQBP1, U5-15kD and U5-52kD.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-27
Detector	MARRESEARCH
Spacegroup name	I 1 2 1
Unit cell lengths	144.180, 40.470, 168.171
Unit cell angles	90.00, 95.92, 90.00

Refinement procedure

Resolution	41.818 - 2.500
R-factor	0.2399
Rwork	0.238
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1syx
RMSD bond length	0.008
RMSD bond angle	1.192
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.270	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.240	0.610
Number of reflections	34203
<I/σ(I)>	4.1	1.8
Completeness [%]	100.0	100
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1