4BWQ
Crystal structure of U5-15kD in a complex with PQBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-07 |
| Detector | MARRSEARCH |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 77.220, 40.040, 118.770 |
| Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
| Resolution | 38.540 - 2.100 |
| R-factor | 0.1893 |
| Rwork | 0.186 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.013 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.520 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.450 |
| Number of reflections | 42475 | |
| <I/σ(I)> | 8.5 | 2.6 |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
