4BVY
Crystal structure of the AIMP3-MRS N-terminal domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.175, 71.250, 64.338 |
| Unit cell angles | 90.00, 104.83, 90.00 |
Refinement procedure
| Resolution | 39.671 - 1.992 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.580 |
| Number of reflections | 25597 | |
| <I/σ(I)> | 25.01 | 4.3 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 6.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 20% PEG 8000, 100 MM HEPES, PH 7.5 |
