4BVU
Structure of Shigella effector OspG in complex with host UbcH5c- Ubiquitin conjugate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 287 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 183.873, 183.873, 63.973 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.160 - 2.700 |
R-factor | 0.24658 |
Rwork | 0.244 |
R-free | 0.29062 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x23 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.919 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.200 | 1.000 |
Number of reflections | 17947 | |
<I/σ(I)> | 16.39 | 2.58 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 19.1 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M TRIS PH 8.5, 20% ETOH |