4BV0
High Resolution Structure of Evolved Agonist-bound Neurotensin Receptor 1 Mutant without Lysozyme Fusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.580, 91.560, 208.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 3.100 |
| Rwork | 0.284 |
| R-free | 0.31050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zev |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.651 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | |
| Number of reflections | 21665 | |
| <I/σ(I)> | 15.55 | 0.51 |
| Completeness [%] | 99.2 | 97 |
| Redundancy | 6.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.4 | 22.5% (V/V) PEG600, 0.5 M NACL, 50 MM GLYCINE PH 9.4 |
