4BSK
Crystal structure of VEGF-C in complex with VEGFR-3 domains D1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 166.660, 166.660, 166.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 4.201 |
| R-factor | 0.338 |
| Rwork | 0.334 |
| R-free | 0.37180 |
| Structure solution method | MIRAS |
| Starting model (for MR) | PDB ENTRIES 2X1X 1FLT AND 2E9W |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.400 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.050 | 1.490 |
| Number of reflections | 5765 | |
| <I/σ(I)> | 20.9 | 2.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.9 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | SITTING DROPS AT ROOM TEMPERATURE OVER A RESERVOIR SOLUTION OF 0.1 M BIS-TRIS BUFFER AT PH 8.5-9.5 AND 1.0-1.5 M AMMONIUM SULPHATE. |
