4BQ7

Crystal structure of the RGMB-Neo1 complex form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 32 1 2
Unit cell lengths	109.690, 109.690, 187.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.779 - 6.601
R-factor	0.2561
Rwork	0.254
R-free	0.27990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.934
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	6.800
High resolution limit [Å]	6.600	6.600
Rmerge	0.230	0.790
Number of reflections	2508
<I/σ(I)>	7.5	2.3
Completeness [%]	97.7	97.4
Redundancy	6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.1 M TRIS-HCL, PH 8.5, 0.2 M LITHIUM SULPHATE, 25 % PEG3350