4BOC

Structure of mitochondrial RNA polymerase elongation complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-25
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 3
Unit cell lengths	225.190, 225.190, 225.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.810 - 2.650
R-factor	0.1808
Rwork	0.179
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3spa
RMSD bond length	0.009
RMSD bond angle	1.030
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.800	2.720
High resolution limit [Å]	2.650	2.650
Rmerge	0.120
Number of reflections	54985
<I/σ(I)>	18.9	1.7
Completeness [%]	100.0	100
Redundancy	20.7	20.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		8% PEG 4000, 200 MM SODIUM ACETATE, 100 MM TRISODIUM CITRATE (PH=5.5), 10% GLYCEROL, 10 MM DTT, pH 7.5