4BL8

Crystal structure of full-length human Suppressor of fused (SUFU)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-07
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	97.320, 99.550, 192.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.666 - 3.040
R-factor	0.2029
Rwork	0.200
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3D4G AND 1M1L
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.780	3.200
High resolution limit [Å]	3.040	3.040
Rmerge	0.120
Number of reflections	36846
<I/σ(I)>	17	2
Completeness [%]	99.9	100
Redundancy	10.9	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6		PROTEIN (12 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4OC BY HANGING DROP VAPOUR DIFFUSION WITH 0.2 M K/NA TARTRATE, 0.1 M BIS-TRIS PROPANE PH 8.5 AND 16% (V/V) PEG 3350 (AT A PROTEIN:MOTHER LIQUOR RATIO OF 2:1)