4BL0
Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.740, 57.900, 118.670 |
| Unit cell angles | 90.00, 102.53, 90.00 |
Refinement procedure
| Resolution | 46.882 - 1.950 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 213S |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.964 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 1.050 |
| Number of reflections | 66488 | |
| <I/σ(I)> | 14.15 | 3 |
| Completeness [%] | 98.7 | 88.1 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M K/ NA TARTRATE, 0.1M BIS TRIS PROPANE PH 7.5, 20% PEG 3350 |
