4BKP
Crystal structure of human GDP-L-fucose synthase with bound NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 106.596, 163.095, 197.581 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.440 - 2.700 |
R-factor | 0.1978 |
Rwork | 0.196 |
R-free | 0.23091 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b8w |
RMSD bond length | 0.007 |
RMSD bond angle | 1.198 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.790 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.100 | 1.070 |
Number of reflections | 47605 | |
<I/σ(I)> | 14.9 | 1.9 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 6.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 3350, 0.1M CITRATE PH 5.5 |