4BKA

crystal structure of the human EphA4 ectodomain in complex with human ephrin A5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Spacegroup name	H 3 2
Unit cell lengths	202.628, 202.628, 326.225
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.870 - 5.300
R-factor	0.38431
Rwork	0.383
R-free	0.40753
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bk5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	5.000
High resolution limit [Å]	4.950	4.950
Rmerge	0.740
Number of reflections	11758
<I/σ(I)>	3.32	0.32
Completeness [%]	99.7	98.5
Redundancy	9.4	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.01 M MGSO4, 1.8 M LISO4, 0.05 M SODIUM CACODYLATE PH 6 AND ADDITIVE 4% BENZAMIDINE-HCL
1				0.01 M MGSO4, 1.8 M LISO4, 0.05 M SODIUM CACODYLATE PH 6 AND ADDITIVE 4% BENZAMIDINE-HCL
1				0.01 M MGSO4, 1.8 M LISO4, 0.05 M SODIUM CACODYLATE PH 6 AND ADDITIVE 4% BENZAMIDINE-HCL