4BHK
Crystal Structure of Moss Leafy bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.722, 84.523, 152.424 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.550 - 2.320 |
| R-factor | 0.1984 |
| Rwork | 0.196 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vy2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 24528 | |
| <I/σ(I)> | 10.08 | 2.64 |
| Completeness [%] | 99.1 | 97.2 |
| Redundancy | 3.78 | 3.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 20 %(V/V) MPD, 0.05 M MES, PH 5.6, 0.1 M MAGNESIUM ACETATE |
