4BDQ
Crystal structure of the GluK2 R775A LBD dimer in complex with glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 95.753, 105.585, 114.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.644 - 1.900 |
R-factor | 0.1679 |
Rwork | 0.166 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xxr |
RMSD bond length | 0.007 |
RMSD bond angle | 0.985 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.640 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.820 |
Number of reflections | 45850 | |
<I/σ(I)> | 14.6 | 1.9 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 21% PEG 4,000, 80MM NA ACETATE | |||
1 | 21% PEG 4,000, 80MM NA ACETATE |