4BA1
Archaeal exosome (Rrp4-Rrp41(D182A)-Rrp42) bound to inorganic phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 135.070, 135.070, 135.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.750 - 1.800 |
| R-factor | 0.18576 |
| Rwork | 0.185 |
| R-free | 0.22171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2je6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.650 |
| Number of reflections | 71709 | |
| <I/σ(I)> | 11.4 | 1.6 |
| Completeness [%] | 1.0 | 0.9 |
| Redundancy | 5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 40% PEG 400, 50MM TRIS HCL PH 8.0 |
