4B9N

Structure of the high fidelity DNA polymerase I correctly bypassing the oxidative formamidopyrimidine-dA DNA lesion.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-08-18
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	88.452, 93.726, 105.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.892 - 2.200
R-factor	0.1849
Rwork	0.183
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b9m
RMSD bond length	0.006
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.890	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.590
Number of reflections	45055
<I/σ(I)>	16	3.2
Completeness [%]	99.8	99.1
Redundancy	4.9	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1