4B8E
PRY-SPRY domain of Trim25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.149, 73.561, 91.009 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.505 - 1.779 |
| R-factor | 0.1744 |
| Rwork | 0.173 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wl1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.010 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.050 | 0.610 |
| Number of reflections | 38142 | |
| <I/σ(I)> | 20.9 | 3.5 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 0.2M NACL, 0.1M TRIS-HCL PH 8.5 |
