4B7I
Crystal Structure of Human IgG Fc Bearing Hybrid-type Glycans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77.2 |
| Detector technology | CCD |
| Collection date | 2007-11-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.041, 72.991, 140.833 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.510 - 2.360 |
| R-factor | 0.21784 |
| Rwork | 0.215 |
| R-free | 0.26613 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ave |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.161 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.070 | 0.470 |
| Number of reflections | 21447 | |
| <I/σ(I)> | 16.5 | 2.4 |
| Completeness [%] | 99.3 | 98.7 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 20% W/V PEG 3350 PH 5.5, 0.2 M SODIUM/POTASSIUM PHOSPHATE |
