4B2Z
Structure of Osh6 in complex with phosphatidylserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-16 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.000, 72.600, 122.900 |
| Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
| Resolution | 46.566 - 1.950 |
| R-factor | 0.1982 |
| Rwork | 0.198 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zht |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.219 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.650 |
| Number of reflections | 65896 | |
| <I/σ(I)> | 18 | 1.6 |
| Completeness [%] | 91.8 | 59 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M MES PH6.5, 13% PEG6000, 5% MPD |
