4B2O
Crystal structure of Bacillus subtilis YmdB, a global regulator of late adaptive responses.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.855, 105.997, 77.920 |
| Unit cell angles | 90.00, 108.30, 90.00 |
Refinement procedure
| Resolution | 19.738 - 1.640 |
| R-factor | 0.1603 |
| Rwork | 0.159 |
| R-free | 0.18540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t70 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.740 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 134017 | |
| <I/σ(I)> | 14.4 | 1.9 |
| Completeness [%] | 96.4 | 79.6 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM ACETATE, 20% PEG 3350 |
