4B1G
Structure of unliganded human PARG catalytic domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-19 |
Detector | ADSC CCD |
Wavelength(s) | 0.979, 0.975, 0.982 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.759, 66.387, 89.323 |
Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
Resolution | 66.430 - 1.830 |
R-factor | 0.18492 |
Rwork | 0.183 |
R-free | 0.21794 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.015 |
RMSD bond angle | 1.444 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.990 | 1.930 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.110 | 0.380 |
Number of reflections | 39497 | |
<I/σ(I)> | 12.4 | 2 |
Completeness [%] | 86.3 | 47.9 |
Redundancy | 6.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 28% PEG3350, 0.2M MAGNESIUM CHLORIDE, 0.1M PCTP (0.04M SODIUM PROPIONATE, 0.02M SODIUM CACODYLATE, 0.04M BIS-TRIS PROPANE) PH 7.5; 1:1 RATIO; 4 MICROLITRE DROP; ROOM TEMPERATURE |