4AZD
T57V mutant of aspartate decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-22 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 69.920, 69.920, 217.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.460 - 1.620 |
| R-factor | 0.18681 |
| Rwork | 0.185 |
| R-free | 0.21709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ppy |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.460 | 1.760 |
| High resolution limit [Å] | 1.620 | 1.670 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 38530 | |
| <I/σ(I)> | 7.4 | 1.4 |
| Completeness [%] | 97.8 | 99.9 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 292 | 2.4 M SODIUM MALONATE, 1.5 M (NH4)2SO4 PH 4, 292 K |
