4AYB
RNAP at 3.2Ang
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 195.725, 212.415, 128.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.097 - 3.202 |
| R-factor | 0.2454 |
| Rwork | 0.242 |
| R-free | 0.30050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2waq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.762 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.100 | 3.270 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.180 | |
| Number of reflections | 88449 | |
| <I/σ(I)> | 13.9 | 2 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 15.2 | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
