4AY9
Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.716, 95.478, 95.675 |
| Unit cell angles | 60.30, 80.02, 75.35 |
Refinement procedure
| Resolution | 47.900 - 2.500 |
| R-factor | 0.23683 |
| Rwork | 0.235 |
| R-free | 0.26901 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xwd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-3000 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 70869 | |
| <I/σ(I)> | 15.8 | 3.3 |
| Completeness [%] | 97.4 | 95.8 |
| Redundancy | 6.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 10% (V/V) ISOPROPANOL AND 20% (W/V) POLYETHYLENE GLYCOL 4000 |
