4AXV
Biochemical and structural characterization of the MpaA amidase as part of a conserved scavenging pathway for peptidoglycan derived peptides in gamma-proteobacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 73.600, 73.600, 208.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.960 - 2.170 |
| R-factor | 0.19761 |
| Rwork | 0.196 |
| R-free | 0.23293 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.038 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELX (CDE) |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.570 | 2.230 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.100 | 0.780 |
| Number of reflections | 18606 | |
| <I/σ(I)> | 33.5 | 7.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 40.8 | 42.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
