4AWN
Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-04-25 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.960, 58.660, 96.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.125 - 1.950 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dni |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.428 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.570 |
| Number of reflections | 18022 | |
| <I/σ(I)> | 6.88 | 1.32 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.48 | 7.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROP VAPOR DIFFUSION, 0.2 M MGCL2, 0.1 M BIS-TRIS AT PH 5.5, 25% V/V PEG 3350 |
