4AW5
Complex of the EphB4 kinase domain with an oxindole inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 93.2 |
| Detector technology | CCD |
| Collection date | 2002-10-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.657, 53.978, 63.242 |
| Unit cell angles | 90.00, 111.06, 90.00 |
Refinement procedure
| Resolution | 58.720 - 2.330 |
| R-factor | 0.19439 |
| Rwork | 0.190 |
| R-free | 0.27894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE APO STRUCTURE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.938 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.120 | 0.460 |
| Number of reflections | 12716 | |
| <I/σ(I)> | 11.4 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.0 |
