4ASA
The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 74.480, 74.480, 111.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.480 - 2.250 |
| R-factor | 0.18205 |
| Rwork | 0.179 |
| R-free | 0.23452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE MODEL |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.218 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.480 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.050 | 0.640 |
| Number of reflections | 15425 | |
| <I/σ(I)> | 4.2 | 2.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
