4AQ1
Structure of the SbsB S-layer protein of Geobacillus stearothermophilus PV72p2 in complex with nanobody KB6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.500, 88.500, 95.200 |
| Unit cell angles | 112.50, 101.60, 84.70 |
Refinement procedure
| Resolution | 43.810 - 2.420 |
| R-factor | 0.18565 |
| Rwork | 0.183 |
| R-free | 0.23567 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.500 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.650 |
| Number of reflections | 74487 | |
| <I/σ(I)> | 11 | 2.5 |
| Completeness [%] | 97.9 | 97.3 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M KSCN, 0.1 M BIS-TRIS PROPANE PH 6.5 AND 20% PEG 3350 |
