4APC
Crystal Structure of Human NIMA-related Kinase 1 (NEK1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Collection date | 2012-02-11 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.910, 92.810, 163.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.970 - 2.100 |
| R-factor | 0.21625 |
| Rwork | 0.215 |
| R-free | 0.24768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jav |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.266 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.410 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.690 |
| Number of reflections | 40349 | |
| <I/σ(I)> | 8.5 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M AMMONIUM CHLORIDE, 20 %(W/V) PEG 3350, pH 7.5 |
