4AP4

Rnf4 - ubch5a - ubiquitin heterotrimeric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-31
Detector	RIGAKU SATURN 944
Spacegroup name	P 21 21 21
Unit cell lengths	50.480, 65.090, 189.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.820 - 2.210
R-factor	0.20926
Rwork	0.207
R-free	0.24175
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2YH0 1UBQ 2XEU
RMSD bond length	0.008
RMSD bond angle	1.248
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER (FOR MR)
Refinement software	REFMAC (5.6.0119)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	190.000	2.260
High resolution limit [Å]	2.210	2.210
Rmerge	0.030	0.170
Number of reflections	32159
<I/σ(I)>	43.9
Completeness [%]	99.2	92
Redundancy	10.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2		pH 7.2