4AP0
The mitotic kinesin Eg5 in complex with Mg-ADP and ispinesib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.723, 112.607, 106.913 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 29.862 - 2.594 |
| R-factor | 0.2065 |
| Rwork | 0.203 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gm1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.469 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 45511 | |
| <I/σ(I)> | 13.3 | 2 |
| Completeness [%] | 95.7 | 91 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 277 | 0.1M TRIS, PH 8.5; 0.02M MGCL2; 20%(W/V) POLYETHYLENE GLYCOL 8000; 4DEGC |
