4AML
CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ ISOLECTIN 1 IN COMPLEX WITH GLYCOSYLURETHAN
Replaces: 2UWGExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.250, 63.250, 153.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.780 - 1.600 |
| R-factor | 0.19819 |
| Rwork | 0.196 |
| R-free | 0.23194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wga |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.119 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.470 |
| Number of reflections | 46032 | |
| <I/σ(I)> | 17.38 | 3.52 |
| Completeness [%] | 96.1 | 89.8 |
| Redundancy | 9.53 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.04 M POTASSIUM PHOSPHATE 14 % PEG 8000 20 % GLYCEROL |
